3QS9

Crystal structure of a human Flt3 ligand-receptor ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-01
Detector	ADSC QUANTUM 315r
Spacegroup name	P 1 21 1
Unit cell lengths	124.750, 153.550, 133.870
Unit cell angles	90.00, 94.57, 90.00

Refinement procedure

Resolution	35.000 - 7.800
R-factor	0.3376
Rwork	0.337
R-free	0.34620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1ETE 2e9w 3qs7
RMSD bond length	0.012
RMSD bond angle	1.360
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER-TNT (BUSTER 2.8.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	8.000
High resolution limit [Å]	7.800	7.800
Rmerge	0.128	0.807
Number of reflections	5656
<I/σ(I)>	9.08	1.9
Completeness [%]	96.6	92.9
Redundancy	3.38	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	12-14% w/v PEG3350, 0.2 M lithium citrate, 0.1 M TRIS-HCl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K