3QL0
Crystal structure of N23PP/S148A mutant of E. coli dihydrofolate reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2010-03-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 79.662, 79.662, 71.464 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.630 - 1.600 |
| R-factor | 0.21029 |
| Rwork | 0.208 |
| R-free | 0.24534 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.539 |
| Data reduction software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.642 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 35547 | |
| Completeness [%] | 99.6 | 97.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.2 M calcium acetate, 0.1 M HEPES, 40% v/v PEG 400, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
