3QE9
Crystal structure of human exonuclease 1 Exo1 (D173A) in complex with DNA (complex I)
Experimental procedure
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-08 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97937 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 92.504, 95.467, 99.777 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.510 |
Rwork | 0.215 |
R-free | 0.23769 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.843 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.780 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 27346 | |
Completeness [%] | 93.3 | 62.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | PEG6K, HEPES, CaCl2, KCl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG6K | ||
2 | 1 | 1 | HEPES | ||
3 | 1 | 1 | CaCl2 | ||
4 | 1 | 1 | KCl | ||
5 | 1 | 2 | PEG6K | ||
6 | 1 | 2 | HEPES | ||
7 | 1 | 2 | CaCl2 | ||
8 | 1 | 2 | KCl |