3PSE
Structure of a viral OTU domain protease bound to interferon-stimulated gene 15 (ISG15)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-06 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.740, 69.810, 69.670 |
| Unit cell angles | 90.00, 93.62, 90.00 |
Refinement procedure
| Resolution | 34.671 - 2.300 |
| R-factor | 0.1666 |
| Rwork | 0.164 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1Z2M AND 3PT2 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.944 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.900 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.107 | 0.566 |
| Number of reflections | 14495 | |
| <I/σ(I)> | 7.4 | 2.3 |
| Completeness [%] | 97.8 | 98 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | 100 mM MES, 23% PEG6000, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
