3PSC
Bovine GRK2 in complex with Gbetagamma subunits
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 115 |
Detector technology | CCD |
Collection date | 2009-11-02 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 187.928, 73.152, 122.780 |
Unit cell angles | 90.00, 115.42, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.670 |
R-factor | 0.22453 |
Rwork | 0.223 |
R-free | 0.25690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1omw |
RMSD bond length | 0.009 |
RMSD bond angle | 1.160 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC (5.5.0109) |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.760 |
High resolution limit [Å] | 2.660 | 2.670 |
Number of reflections | 43102 | |
<I/σ(I)> | 15.7 | 2.6 |
Completeness [%] | 99.6 | 99.8 |
Redundancy | 4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 9% PEG3350, 200 mM NaCl, 100 mM MES pH 6.5, 1 mM ATP, VAPOR DIFFUSION, HANGING DROP, temperature 277K |