3PSC

Bovine GRK2 in complex with Gbetagamma subunits

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Beta-adrenergic receptor kinase 1	polymer	695	80578.8	1	UniProt (P21146) Pfam (PF00615) Pfam (PF00069) Pfam (PF00169)	Bos taurus (bovine)	GRK2, Beta-ARK-1, G-protein-coupled receptor kinase 2
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37416.9	1	UniProt (P62871) Pfam (PF00400)	Bos taurus (bovine)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	74	8406.7	1	UniProt (P63212) Pfam (PF00631)	Bos taurus (bovine)	G gamma-I
4	D, E, F (A, B, G)	water	water		18.0	8	Chemie (HOH)

Sequence modifications

A: 1 - 689 (UniProt: P21146)

PDB	External Database	Details
Ala 670	Ser 670	engineered mutation
His 690	-	expression tag
His 691	-	expression tag
His 692	-	expression tag
His 693	-	expression tag
His 694	-	expression tag
His 695	-	expression tag

G: 1 - 68 (UniProt: P63212)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	126402.4
All*	Total formula weight	126402.4

*Water molecules are not included.