3PFV
Crystal structure of Cbl-b TKB domain in complex with EGFR pY1069 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.063, 98.934, 61.978 |
| Unit cell angles | 90.00, 110.63, 90.00 |
Refinement procedure
| Resolution | 49.470 - 2.270 |
| R-factor | 0.21861 |
| Rwork | 0.216 |
| R-free | 0.26201 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 3BUO |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.567 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.470 | 2.390 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.090 | 0.604 |
| Number of reflections | 30186 | |
| <I/σ(I)> | 8 | 2.1 |
| Completeness [%] | 96.6 | 96.6 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.15 | 30% PEG 3350, 0.3M Ammonium sulfate, 0.1M Bis-Tris, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |
