3PD8
X-ray structure of the ligand-binding core of GluA2 in complex with (S)-7-HPCA at 2.5 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2006-04-26 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.043 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 114.618, 164.002, 47.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.320 - 2.476 |
| R-factor | 0.1798 |
| Rwork | 0.175 |
| R-free | 0.26050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m5e |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.947 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.590 |
| High resolution limit [Å] | 2.476 | 5.380 | 2.476 |
| Rmerge | 0.114 | 0.077 | 0.398 |
| Number of reflections | 32227 | ||
| <I/σ(I)> | 7.4 | ||
| Completeness [%] | 98.3 | 95 | 97.8 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 280 | PEG4000, zinc acetate, cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 280K |
