3P57
Crystal structure of the p300 TAZ2 domain bound to MEF2 on DNA
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.846, 90.926, 144.802 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.666 - 2.192 |
R-factor | 0.2247 |
Rwork | 0.219 |
R-free | 0.27240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1egw |
RMSD bond length | 0.008 |
RMSD bond angle | 1.324 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.160 |
High resolution limit [Å] | 2.090 | 2.090 |
Rmerge | 0.087 | 0.709 |
Number of reflections | 59524 | |
<I/σ(I)> | 27.3 | 1.95 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 288 | 16% PEG 1000, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 288K |