3O0G
Crystal Structure of Cdk5:p25 in complex with an ATP analogue
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-01-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 117.124, 117.124, 155.599 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.960 - 1.950 |
| R-factor | 0.22763 |
| Rwork | 0.226 |
| R-free | 0.25626 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h4l |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.180 |
| Data reduction software | DENZO |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 88794 | |
| <I/σ(I)> | 17.3 | 1.8 |
| Completeness [%] | 98.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | PEG 3350, KI, Bis-Tris Propane, pH 7, vapor diffusion, temperature 293K |
