3NJP
The Extracellular and Transmembrane Domain Interfaces in Epidermal Growth Factor Receptor Signaling
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Wavelength(s) | 1.0000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 220.167, 220.167, 113.123 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.493 - 3.304 |
R-factor | 0.2649 |
Rwork | 0.263 |
R-free | 0.29790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ivo |
RMSD bond length | 0.002 |
RMSD bond angle | 0.541 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.420 |
High resolution limit [Å] | 3.300 | 7.100 | 3.300 |
Rmerge | 0.078 | 0.051 | 0.358 |
Rmeas | 0.078 | ||
Total number of observations | 320883 | ||
Number of reflections | 46667 | ||
<I/σ(I)> | 11.6 | ||
Completeness [%] | 98.2 | 99.6 | 92.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293 | 15% PEG4000, 1% PEG6000, 75 mM Tris-HCl, 75 mM soidum acetate, and 200 mM NaCl, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |