1IVO

Crystal Structure of the Complex of Human Epidermal Growth Factor and Receptor Extracellular Domains.

Summary for 1IVO

• Entry DOI: 10.2210/pdb1ivo/pdb
• Related: 1JL9
• Descriptor: Epidermal Growth Factor Receptor, Epidermal growth factor, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• Functional Keywords: transmembrane, glycoprotein, receptor, riken structural genomics/proteomics initiative, rsgi, structural genomics, transferase-signaling protein complex, transferase/signaling protein
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 4
• Total formula weight: 152212.77

Authors

Ogiso, H.,Ishitani, R.,Nureki, O.,Fukai, S.,Yamanaka, M.,Kim, J.H.,Saito, K.,Shirouzu, M.,Yokoyama, S.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (deposition date: 2002-03-28, release date: 2002-10-16, Last modification date: 2024-10-23)

Primary citation

Ogiso, H.,Ishitani, R.,Nureki, O.,Fukai, S.,Yamanaka, M.,Kim, J.H.,Saito, K.,Inoue, M.,Shirouzu, M.,Yokoyama, S.
Crystal Structure of the Complex of Human Epidermal Growth Factor and Receptor Extracellular Domains.
Cell(Cambridge,Mass.), 110:775-787, 2002

PubMed Abstract: Epidermal growth factor (EGF) regulates cell proliferation and differentiation by binding to the EGF receptor (EGFR) extracellular region, comprising domains I-IV, with the resultant dimerization of the receptor tyrosine kinase. In this study, the crystal structure of a 2:2 complex of human EGF and the EGFR extracellular region has been determined at 3.3 A resolution. EGFR domains I-III are arranged in a C shape, and EGF is docked between domains I and III. The 1:1 EGF*EGFR complex dimerizes through a direct receptor*receptor interaction, in which a protruding beta-hairpin arm of each domain II holds the body of the other. The unique "receptor-mediated dimerization" was verified by EGFR mutagenesis.

PubMed: 12297050
DOI: 10.1016/S0092-8674(02)00963-7

Experimental method

X-RAY DIFFRACTION (3.3 Å)