1IVO
Crystal Structure of the Complex of Human Epidermal Growth Factor and Receptor Extracellular Domains.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 220.164, 220.164, 113.121 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 3.300 |
| R-factor | 0.258 |
| Rwork | 0.255 |
| R-free | 0.32600 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.390 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.710 |
| High resolution limit [Å] | 3.300 * | 3.500 |
| Rmerge | 0.078 * | 0.358 * |
| Total number of observations | 320883 * | |
| Number of reflections | 46667 * | |
| <I/σ(I)> | 25.6 | 5.1 |
| Completeness [%] | 98.2 * | 92.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 20 * | PEG4000, PEG6000, sodium acetate, sodium chloride, TRIS, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 20 * | PEG4000, PEG6000, sodium acetate, sodium chloride, TRIS, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 15 (%) | |
| 2 | 1 | reservoir | PEG6000 | 1 (%) | |
| 3 | 1 | reservoir | Tris-HCl | 75 (mM) | pH8.4 |
| 4 | 1 | reservoir | sodium acetate | 75 (mM) | |
| 5 | 1 | reservoir | 200 (mM) | ||
| 6 | 1 | drop | protein | 10 (mg/ml) |
