3NBN

Crystal structure of a dimer of Notch Transcription Complex trimers on HES1 DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97926
Spacegroup name	C 1 2 1
Unit cell lengths	295.112, 108.059, 87.239
Unit cell angles	90.00, 102.52, 90.00

Refinement procedure

Resolution	45.020 - 3.450
R-factor	0.256
Rwork	0.254
R-free	0.29800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	protein components of 2F8X
RMSD bond length	0.014
RMSD bond angle	1.609
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.510
High resolution limit [Å]	3.450	9.350	3.450
Rmerge	0.063	0.029	0.396
Number of reflections	35389
<I/σ(I)>	12.4
Completeness [%]	99.9	98.8	99.9
Redundancy	9.5	9.9	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	3% PEG3350, 10% ethylene glycol, 0.15M NaCl, 0.1M magnesium chloride, 0.1M BIS-TRIS, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 298K