3MYG
Aurora A Kinase complexed with SCH 1473759
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-09-27 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 80.880, 80.880, 171.282 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.420 - 2.400 |
| R-factor | 0.2224 |
| Rwork | 0.221 |
| R-free | 0.24490 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1mq4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.090 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | BUSTER (2.9.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.064 | 0.653 |
| Number of reflections | 13705 | |
| <I/σ(I)> | 14.5 | 2.3 |
| Completeness [%] | 91.3 | |
| Redundancy | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 291 | 0.1M Tris, 0.2M LiSO4, 33% PEG 400, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
