3MB3
Crystal Structure of the second bromodomain of Pleckstrin homology domain interacting protein (PHIP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Collection date | 2010-03-03 |
Wavelength(s) | 0.9762 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 92.090, 92.090, 76.120 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.880 - 2.250 |
R-factor | 0.208 |
Rwork | 0.206 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Ensemble of 3HMH 2nxb 2oo1 2oss 2ouo 2rfj 3dai 3d7c 3dwy |
RMSD bond length | 0.016 |
RMSD bond angle | 1.573 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.880 | 2.370 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.081 | 0.453 |
Number of reflections | 18012 | |
<I/σ(I)> | 9.2 | 2 |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 3.6 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 277 | 11% PEG3350 4% glycerol,0.1M acetate pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |