3MB3
Crystal Structure of the second bromodomain of Pleckstrin homology domain interacting protein (PHIP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Collection date | 2010-03-03 |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 92.090, 92.090, 76.120 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.880 - 2.250 |
| R-factor | 0.208 |
| Rwork | 0.206 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Ensemble of 3HMH 2nxb 2oo1 2oss 2ouo 2rfj 3dai 3d7c 3dwy |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.573 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.880 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.081 | 0.453 |
| Number of reflections | 18012 | |
| <I/σ(I)> | 9.2 | 2 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 277 | 11% PEG3350 4% glycerol,0.1M acetate pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
