3M59
SET7/9 Y245A in complex with TAF10-K189me2 peptide and AdoHcy
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-02 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9786 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 83.497, 83.497, 95.718 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.160 - 1.700 |
R-factor | 0.188 |
Rwork | 0.187 |
R-free | 0.20600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2f69 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.522 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 1.760 |
High resolution limit [Å] | 1.700 | 3.660 | 1.700 |
Rmerge | 0.055 | 0.031 | 0.527 |
Number of reflections | 40597 | ||
<I/σ(I)> | 15.1 | ||
Completeness [%] | 95.0 | 90.3 | 88.1 |
Redundancy | 6 | 6.5 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | 1.9 M Ammonium Sulfate, 0.1 M Bis-Tris pH 6.4, 0.005 M CoCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |