3LTG
Crystal structure of the Drosophila Epidermal Growth Factor Receptor ectodomain complexed with a low affinity Spitz mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-10 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97928 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.750, 120.150, 274.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.400 |
R-factor | 0.4077 |
Rwork | 0.406 |
R-free | 0.42675 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3I2T and 3LTF; s-dEGFR ectodomain unliganded and complexed with Spitz |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.570 |
High resolution limit [Å] | 3.400 | 3.400 |
Number of reflections | 28590 | |
<I/σ(I)> | 17 | 2.1 |
Completeness [%] | 99.0 | 97.2 |
Redundancy | 7 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 294 | 11% PEG 20,000 0.1M HEPES pH7.4 3% n-propanol, VAPOR DIFFUSION, HANGING DROP, temperature 294K |