3LO5
Crystal Structure of the dominant negative S17N mutant of Ras
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 92.475, 102.184, 117.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.810 - 2.568 |
| R-factor | 0.2406 |
| Rwork | 0.238 |
| R-free | 0.29640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4q21 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.734 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.700 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.100 | 0.580 |
| Number of reflections | 17942 | |
| <I/σ(I)> | 17.5 | 3.2 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 5.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 15% (v/v) PEG400, 13% (w/v) PEG8000, 0.2 M calcium acetate, and 0.1 M Tris-HCl pH = 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
