3LO5

Crystal Structure of the dominant negative S17N mutant of Ras

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003924	molecular_function	GTPase activity
A	0005525	molecular_function	GTP binding
A	0007165	biological_process	signal transduction
A	0016020	cellular_component	membrane
C	0003924	molecular_function	GTPase activity
C	0005525	molecular_function	GTP binding
C	0007165	biological_process	signal transduction
C	0016020	cellular_component	membrane
E	0003924	molecular_function	GTPase activity
E	0005525	molecular_function	GTP binding
E	0007165	biological_process	signal transduction
E	0016020	cellular_component	membrane

Functional Information from PDB Data

site_id	AC1
Number of Residues	16
Details	BINDING SITE FOR RESIDUE GDP A 180

Chain	Residue
A	GLY12
A	LYS117
A	ASP119
A	LEU120
A	SER145
A	ALA146
A	LYS147
A	CA167
A	GLY13
A	VAL14
A	GLY15
A	LYS16
A	ASN17
A	ALA18
A	PHE28
A	ASN116

---

site_id	AC2
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CA A 167

Chain	Residue
A	GDP180

---

site_id	AC3
Number of Residues	20
Details	BINDING SITE FOR RESIDUE GDP E 180

Chain	Residue
E	GLY12
E	GLY13
E	VAL14
E	GLY15
E	LYS16
E	ASN17
E	ALA18
E	PHE28
E	ASP30
E	ASP33
E	ASN116
E	LYS117
E	ASP119
E	LEU120
E	SER145
E	ALA146
E	LYS147
E	CA167
E	HOH176
E	HOH181

---

site_id	AC4
Number of Residues	15
Details	BINDING SITE FOR RESIDUE GDP C 180

Chain	Residue
C	GLY13
C	VAL14
C	GLY15
C	LYS16
C	ASN17
C	ALA18
C	ASN116
C	LYS117
C	ASP119
C	LEU120
C	SER145
C	ALA146
C	LYS147
C	CA168
C	HOH175

---

site_id	AC5
Number of Residues	8
Details	BINDING SITE FOR RESIDUE SO4 C 167

Chain	Residue
A	THR124
A	GLU126
A	GLN129
C	THR124
C	GLU126
C	GLN129
C	HOH183
E	THR124

---

site_id	AC6
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CA C 168

Chain	Residue
C	GDP180

---

site_id	AC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CA E 167

Chain	Residue
E	ASP33
E	HOH176
E	GDP180

---

site_id	AC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CA E 168

Chain	Residue
E	ASP30
E	ASP30
E	GLU31
E	GLU31

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	15
Details	BINDING: BINDING => ECO:0000269|PubMed:16698776

Chain	Residue	Details
A	GLY13
C	SER145
E	GLY13
E	VAL29
E	ALA59
E	ASN116
E	SER145
A	VAL29
A	ALA59
A	ASN116
A	SER145
C	GLY13
C	VAL29
C	ALA59
C	ASN116

---

site_id	SWS_FT_FI2
Number of Residues	3
Details	MOD_RES: N-acetylmethionine; in GTPase HRas; alternate => ECO:0000269|Ref.12

Chain	Residue	Details
A	MET1
C	MET1
E	MET1

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	MOD_RES: N-acetylthreonine; in GTPase HRas, N-terminally processed => ECO:0000269|Ref.12

Chain	Residue	Details
A	THR2
C	THR2
E	THR2

---

site_id	SWS_FT_FI4
Number of Residues	3
Details	MOD_RES: S-nitrosocysteine => ECO:0000269|PubMed:9020151

Chain	Residue	Details
A	CYS118
C	CYS118
E	CYS118

---

site_id	SWS_FT_FI5
Number of Residues	3
Details	CARBOHYD: (Microbial infection) O-linked (Glc) threonine; by P.sordellii toxin TcsL => ECO:0000269|PubMed:19744486, ECO:0000269|PubMed:8626575, ECO:0000269|PubMed:8626586, ECO:0000269|PubMed:9632667

Chain	Residue	Details
A	THR35
C	THR35
E	THR35

---