3LNZ
Crystal structure of human MDM2 with a 12-mer peptide inhibitor PMI (N8A mutant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-01-06 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 90.544, 90.544, 196.837 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.950 |
| R-factor | 0.22186 |
| Rwork | 0.220 |
| R-free | 0.26687 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eqs |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.846 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.413 | 1.980 |
| High resolution limit [Å] | 1.949 | 1.950 |
| Rmerge | 0.119 | 0.575 |
| Number of reflections | 67730 | |
| <I/σ(I)> | 23.1 | 4 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 8.2 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 273 | 0.2 M Mg acetate tetrahydrate sulfate, 0.1 M cacodylate trihydrate, 20% PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
