3KZ1
Crystal Structure of the Complex of PDZ-RhoGEF DH/PH domains with GTP-gamma-S Activated RhoA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-21 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 98.166, 111.836, 138.390 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.450 - 2.700 |
R-factor | 0.23604 |
Rwork | 0.234 |
R-free | 0.28328 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 1A2B 1xcg |
RMSD bond length | 0.014 |
RMSD bond angle | 1.436 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.090 | 0.691 |
Number of reflections | 38512 | |
<I/σ(I)> | 16.5 | 1.46 |
Completeness [%] | 90.7 | 62.1 |
Redundancy | 4.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 14-16% PEG 10000, 0.1M ammonium acetate, 0.1M Bis-Tris, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |