Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3KZ1

Crystal Structure of the Complex of PDZ-RhoGEF DH/PH domains with GTP-gamma-S Activated RhoA

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005085	molecular_function	guanyl-nucleotide exchange factor activity
A	0007186	biological_process	G protein-coupled receptor signaling pathway
A	0007266	biological_process	Rho protein signal transduction
B	0005085	molecular_function	guanyl-nucleotide exchange factor activity
B	0007186	biological_process	G protein-coupled receptor signaling pathway
B	0007266	biological_process	Rho protein signal transduction
E	0000166	molecular_function	nucleotide binding
E	0000902	biological_process	cell morphogenesis
E	0001764	biological_process	neuron migration
E	0001822	biological_process	kidney development
E	0001998	biological_process	angiotensin-mediated vasoconstriction involved in regulation of systemic arterial blood pressure
E	0002363	biological_process	alpha-beta T cell lineage commitment
E	0003100	biological_process	regulation of systemic arterial blood pressure by endothelin
E	0003189	biological_process	aortic valve formation
E	0003924	molecular_function	GTPase activity
E	0003925	molecular_function	G protein activity
E	0005515	molecular_function	protein binding
E	0005525	molecular_function	GTP binding
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005768	cellular_component	endosome
E	0005789	cellular_component	endoplasmic reticulum membrane
E	0005829	cellular_component	cytosol
E	0005856	cellular_component	cytoskeleton
E	0005886	cellular_component	plasma membrane
E	0005925	cellular_component	focal adhesion
E	0005938	cellular_component	cell cortex
E	0006357	biological_process	regulation of transcription by RNA polymerase II
E	0007010	biological_process	cytoskeleton organization
E	0007049	biological_process	cell cycle
E	0007155	biological_process	cell adhesion
E	0007160	biological_process	cell-matrix adhesion
E	0007264	biological_process	small GTPase-mediated signal transduction
E	0007266	biological_process	Rho protein signal transduction
E	0007519	biological_process	skeletal muscle tissue development
E	0009898	cellular_component	cytoplasmic side of plasma membrane
E	0010812	biological_process	negative regulation of cell-substrate adhesion
E	0010975	biological_process	regulation of neuron projection development
E	0016020	cellular_component	membrane
E	0016477	biological_process	cell migration
E	0016787	molecular_function	hydrolase activity
E	0017022	molecular_function	myosin binding
E	0019901	molecular_function	protein kinase binding
E	0021762	biological_process	substantia nigra development
E	0021795	biological_process	cerebral cortex cell migration
E	0021861	biological_process	forebrain radial glial cell differentiation
E	0030027	cellular_component	lamellipodium
E	0030036	biological_process	actin cytoskeleton organization
E	0030054	cellular_component	cell junction
E	0030154	biological_process	cell differentiation
E	0030334	biological_process	regulation of cell migration
E	0030425	cellular_component	dendrite
E	0030496	cellular_component	midbody
E	0030521	biological_process	androgen receptor signaling pathway
E	0030667	cellular_component	secretory granule membrane
E	0031122	biological_process	cytoplasmic microtubule organization
E	0031982	cellular_component	vesicle
E	0032154	cellular_component	cleavage furrow
E	0032467	biological_process	positive regulation of cytokinesis
E	0032587	cellular_component	ruffle membrane
E	0032956	biological_process	regulation of actin cytoskeleton organization
E	0033144	biological_process	negative regulation of intracellular steroid hormone receptor signaling pathway
E	0033688	biological_process	regulation of osteoblast proliferation
E	0034329	biological_process	cell junction assembly
E	0034446	biological_process	substrate adhesion-dependent cell spreading
E	0035385	biological_process	Roundabout signaling pathway
E	0036089	biological_process	cleavage furrow formation
E	0038027	biological_process	apolipoprotein A-I-mediated signaling pathway
E	0042476	biological_process	odontogenesis
E	0042995	cellular_component	cell projection
E	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
E	0043149	biological_process	stress fiber assembly
E	0043197	cellular_component	dendritic spine
E	0043296	cellular_component	apical junction complex
E	0043297	biological_process	apical junction assembly
E	0043366	biological_process	beta selection
E	0043542	biological_process	endothelial cell migration
E	0043931	biological_process	ossification involved in bone maturation
E	0044319	biological_process	wound healing, spreading of cells
E	0045198	biological_process	establishment of epithelial cell apical/basal polarity
E	0045666	biological_process	positive regulation of neuron differentiation
E	0045792	biological_process	negative regulation of cell size
E	0046638	biological_process	positive regulation of alpha-beta T cell differentiation
E	0050919	biological_process	negative chemotaxis
E	0051301	biological_process	cell division
E	0051496	biological_process	positive regulation of stress fiber assembly
E	0051893	biological_process	regulation of focal adhesion assembly
E	0060071	biological_process	Wnt signaling pathway, planar cell polarity pathway
E	0060193	biological_process	positive regulation of lipase activity
E	0061430	biological_process	bone trabecula morphogenesis
E	0070062	cellular_component	extracellular exosome
E	0070507	biological_process	regulation of microtubule cytoskeleton organization
E	0071222	biological_process	cellular response to lipopolysaccharide
E	0071345	biological_process	cellular response to cytokine stimulus
E	0071526	biological_process	semaphorin-plexin signaling pathway
E	0071803	biological_process	positive regulation of podosome assembly
E	0071902	biological_process	positive regulation of protein serine/threonine kinase activity
E	0071944	cellular_component	cell periphery
E	0090051	biological_process	negative regulation of cell migration involved in sprouting angiogenesis
E	0090307	biological_process	mitotic spindle assembly
E	0090324	biological_process	negative regulation of oxidative phosphorylation
E	0097049	biological_process	motor neuron apoptotic process
E	0097498	biological_process	endothelial tube lumen extension
E	0098794	cellular_component	postsynapse
E	0098978	cellular_component	glutamatergic synapse
E	0099159	biological_process	regulation of modification of postsynaptic structure
E	0101003	cellular_component	ficolin-1-rich granule membrane
E	1901224	biological_process	positive regulation of non-canonical NF-kappaB signal transduction
E	1902766	biological_process	skeletal muscle satellite cell migration
E	1903427	biological_process	negative regulation of reactive oxygen species biosynthetic process
E	1903673	biological_process	mitotic cleavage furrow formation
E	1904695	biological_process	positive regulation of vascular associated smooth muscle contraction
E	1904996	biological_process	positive regulation of leukocyte adhesion to vascular endothelial cell
E	1905274	biological_process	regulation of modification of postsynaptic actin cytoskeleton
E	1990869	biological_process	cellular response to chemokine
E	2000177	biological_process	regulation of neural precursor cell proliferation
E	2000406	biological_process	positive regulation of T cell migration
E	2000672	biological_process	negative regulation of motor neuron apoptotic process
F	0000166	molecular_function	nucleotide binding
F	0000902	biological_process	cell morphogenesis
F	0001764	biological_process	neuron migration
F	0001822	biological_process	kidney development
F	0001998	biological_process	angiotensin-mediated vasoconstriction involved in regulation of systemic arterial blood pressure
F	0002363	biological_process	alpha-beta T cell lineage commitment
F	0003100	biological_process	regulation of systemic arterial blood pressure by endothelin
F	0003189	biological_process	aortic valve formation
F	0003924	molecular_function	GTPase activity
F	0003925	molecular_function	G protein activity
F	0005515	molecular_function	protein binding
F	0005525	molecular_function	GTP binding
F	0005634	cellular_component	nucleus
F	0005737	cellular_component	cytoplasm
F	0005768	cellular_component	endosome
F	0005789	cellular_component	endoplasmic reticulum membrane
F	0005829	cellular_component	cytosol
F	0005856	cellular_component	cytoskeleton
F	0005886	cellular_component	plasma membrane
F	0005925	cellular_component	focal adhesion
F	0005938	cellular_component	cell cortex
F	0006357	biological_process	regulation of transcription by RNA polymerase II
F	0007010	biological_process	cytoskeleton organization
F	0007049	biological_process	cell cycle
F	0007155	biological_process	cell adhesion
F	0007160	biological_process	cell-matrix adhesion
F	0007264	biological_process	small GTPase-mediated signal transduction
F	0007266	biological_process	Rho protein signal transduction
F	0007519	biological_process	skeletal muscle tissue development
F	0009898	cellular_component	cytoplasmic side of plasma membrane
F	0010812	biological_process	negative regulation of cell-substrate adhesion
F	0010975	biological_process	regulation of neuron projection development
F	0016020	cellular_component	membrane
F	0016477	biological_process	cell migration
F	0016787	molecular_function	hydrolase activity
F	0017022	molecular_function	myosin binding
F	0019901	molecular_function	protein kinase binding
F	0021762	biological_process	substantia nigra development
F	0021795	biological_process	cerebral cortex cell migration
F	0021861	biological_process	forebrain radial glial cell differentiation
F	0030027	cellular_component	lamellipodium
F	0030036	biological_process	actin cytoskeleton organization
F	0030054	cellular_component	cell junction
F	0030154	biological_process	cell differentiation
F	0030334	biological_process	regulation of cell migration
F	0030425	cellular_component	dendrite
F	0030496	cellular_component	midbody
F	0030521	biological_process	androgen receptor signaling pathway
F	0030667	cellular_component	secretory granule membrane
F	0031122	biological_process	cytoplasmic microtubule organization
F	0031982	cellular_component	vesicle
F	0032154	cellular_component	cleavage furrow
F	0032467	biological_process	positive regulation of cytokinesis
F	0032587	cellular_component	ruffle membrane
F	0032956	biological_process	regulation of actin cytoskeleton organization
F	0033144	biological_process	negative regulation of intracellular steroid hormone receptor signaling pathway
F	0033688	biological_process	regulation of osteoblast proliferation
F	0034329	biological_process	cell junction assembly
F	0034446	biological_process	substrate adhesion-dependent cell spreading
F	0035385	biological_process	Roundabout signaling pathway
F	0036089	biological_process	cleavage furrow formation
F	0038027	biological_process	apolipoprotein A-I-mediated signaling pathway
F	0042476	biological_process	odontogenesis
F	0042995	cellular_component	cell projection
F	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
F	0043149	biological_process	stress fiber assembly
F	0043197	cellular_component	dendritic spine
F	0043296	cellular_component	apical junction complex
F	0043297	biological_process	apical junction assembly
F	0043366	biological_process	beta selection
F	0043542	biological_process	endothelial cell migration
F	0043931	biological_process	ossification involved in bone maturation
F	0044319	biological_process	wound healing, spreading of cells
F	0045198	biological_process	establishment of epithelial cell apical/basal polarity
F	0045666	biological_process	positive regulation of neuron differentiation
F	0045792	biological_process	negative regulation of cell size
F	0046638	biological_process	positive regulation of alpha-beta T cell differentiation
F	0050919	biological_process	negative chemotaxis
F	0051301	biological_process	cell division
F	0051496	biological_process	positive regulation of stress fiber assembly
F	0051893	biological_process	regulation of focal adhesion assembly
F	0060071	biological_process	Wnt signaling pathway, planar cell polarity pathway
F	0060193	biological_process	positive regulation of lipase activity
F	0061430	biological_process	bone trabecula morphogenesis
F	0070062	cellular_component	extracellular exosome
F	0070507	biological_process	regulation of microtubule cytoskeleton organization
F	0071222	biological_process	cellular response to lipopolysaccharide
F	0071345	biological_process	cellular response to cytokine stimulus
F	0071526	biological_process	semaphorin-plexin signaling pathway
F	0071803	biological_process	positive regulation of podosome assembly
F	0071902	biological_process	positive regulation of protein serine/threonine kinase activity
F	0071944	cellular_component	cell periphery
F	0090051	biological_process	negative regulation of cell migration involved in sprouting angiogenesis
F	0090307	biological_process	mitotic spindle assembly
F	0090324	biological_process	negative regulation of oxidative phosphorylation
F	0097049	biological_process	motor neuron apoptotic process
F	0097498	biological_process	endothelial tube lumen extension
F	0098794	cellular_component	postsynapse
F	0098978	cellular_component	glutamatergic synapse
F	0099159	biological_process	regulation of modification of postsynaptic structure
F	0101003	cellular_component	ficolin-1-rich granule membrane
F	1901224	biological_process	positive regulation of non-canonical NF-kappaB signal transduction
F	1902766	biological_process	skeletal muscle satellite cell migration
F	1903427	biological_process	negative regulation of reactive oxygen species biosynthetic process
F	1903673	biological_process	mitotic cleavage furrow formation
F	1904695	biological_process	positive regulation of vascular associated smooth muscle contraction
F	1904996	biological_process	positive regulation of leukocyte adhesion to vascular endothelial cell
F	1905274	biological_process	regulation of modification of postsynaptic actin cytoskeleton
F	1990869	biological_process	cellular response to chemokine
F	2000177	biological_process	regulation of neural precursor cell proliferation
F	2000406	biological_process	positive regulation of T cell migration
F	2000672	biological_process	negative regulation of motor neuron apoptotic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG E 550

Chain	Residue
E	THR19
E	THR37
E	ASP59
E	GSP538

site_id	AC2
Number of Residues	18
Details	BINDING SITE FOR RESIDUE GSP E 538

Chain	Residue
E	THR19
E	CYS20
E	PHE30
E	TYR34
E	THR37
E	GLY62
E	LYS118
E	ASP120
E	LEU121
E	ALA161
E	LYS162
E	HOH207
E	MG550
E	ASP13
E	ALA15
E	CYS16
E	GLY17
E	LYS18

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG F 550

Chain	Residue
F	THR19
F	THR37
F	ASP59
F	THR60
F	GSP538

site_id	AC4
Number of Residues	17
Details	BINDING SITE FOR RESIDUE GSP F 538

Chain	Residue
F	ALA15
F	CYS16
F	GLY17
F	LYS18
F	THR19
F	CYS20
F	PHE30
F	TYR34
F	THR37
F	GLY62
F	LYS118
F	ASP120
F	LEU121
F	ALA161
F	LYS162
F	HOH206
F	MG550

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269\|PubMed:10748207, ECO:0000269\|PubMed:12777804

Chain	Residue	Details
E	GLY12
E	ASN117
F	GLY12
F	ASN117

site_id	SWS_FT_FI2
Number of Residues	6
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P62820

Chain	Residue	Details
E	PHE30
E	ASP59
E	SER160
F	PHE30
F	ASP59
F	SER160

site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: (Microbial infection) O-AMP-tyrosine; by Haemophilus IbpA; alternate => ECO:0000269\|PubMed:19362538

Chain	Residue	Details
E	TYR34
F	TYR34

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: (Microbial infection) O-AMP-threonine; by Vibrio VopS => ECO:0000269\|PubMed:19039103

Chain	Residue	Details
E	THR37
F	THR37

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: (Microbial infection) ADP-ribosylasparagine; by botulinum toxin => ECO:0000305\|PubMed:1328215

Chain	Residue	Details
E	ASN41
F	ASN41

site_id	SWS_FT_FI6
Number of Residues	2
Details	MOD_RES: 5-glutamyl serotonin => ECO:0000250\|UniProtKB:Q9QUI0

Chain	Residue	Details
E	GLN63
F	GLN63

site_id	SWS_FT_FI7
Number of Residues	2
Details	CARBOHYD: (Microbial infection) O-linked (GlcNAc) tyrosine; by Photorhabdus PAU_02230; alternate => ECO:0000269\|PubMed:24141704

Chain	Residue	Details
E	TYR34
F	TYR34

site_id	SWS_FT_FI8
Number of Residues	2
Details	CARBOHYD: (Microbial infection) O-linked (Glc) threonine; by C.difficile toxins TcdA and TcdB; alternate => ECO:0000269\|PubMed:24905543, ECO:0000269\|PubMed:7775453, ECO:0000269\|PubMed:7777059

Chain	Residue	Details
E	THR37
F	THR37

site_id	SWS_FT_FI9
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:23871831

Chain	Residue	Details
E	LYS135
F	LYS135

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)