3KS8
Crystal structure of Reston ebolavirus VP35 RNA binding domain in complex with 18bp dsRNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-12 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.9795 |
Spacegroup name | P 31 |
Unit cell lengths | 85.685, 85.685, 108.812 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.117 - 2.401 |
R-factor | 0.2 |
Rwork | 0.198 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ks4 |
RMSD bond length | 0.021 |
RMSD bond angle | 1.304 |
Data reduction software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_222)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.100 | 2.380 |
High resolution limit [Å] | 2.280 | 2.280 |
Rmerge | 0.061 | 0.254 |
Number of reflections | 33227 | |
<I/σ(I)> | 23.47 | 5.03 |
Completeness [%] | 81.4 | 35.3 |
Redundancy | 4.3 | 1.43 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295.5 | 365mM ammonium acetate, 100mM trisodium citrate, 18.5% PEG 4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.5K |