3KQU
Three Conformational Snapshots of the Hepatitis C Virus NS3 Helicase Reveal a Ratchet Translocation Mechanism
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Detector technology | CCD |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0809 |
Spacegroup name | P 1 |
Unit cell lengths | 116.534, 116.467, 71.114 |
Unit cell angles | 90.00, 90.00, 119.97 |
Refinement procedure
Resolution | 50.000 - 2.400 |
Rwork | 0.197 |
R-free | 0.21500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.315 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.400 |
Rmerge | 0.070 |
Number of reflections | 121925 |
Completeness [%] | 96.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 10 mM NaF, 2.5% (w/v) PEG 4000, and 15% (w/v) PEG MME 550, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |