3KQ6
Enhancing the Therapeutic Properties of a Protein by a Designed Zinc-Binding Site, Structural principles of a novel long-acting insulin analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-04 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.0000,1.2398 |
| Spacegroup name | H 3 |
| Unit cell lengths | 78.091, 78.091, 36.399 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.050 - 1.900 |
| Rwork | 0.199 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rwe |
| RMSD bond angle | 0.008 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.050 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.057 | 0.422 |
| Number of reflections | 6488 | |
| <I/σ(I)> | 14.1 | 3 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 5.49 | 5.43 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 298 | 0.02 M Tris, 0.05 M sodium citrate, 5% acetone, 0.03% phenol, 0.01% zinc acetate, pH 8.0, VAPOR DIFFUSION, temperature 298K |
