3KBU
Crystal structure of the ankyrin binding domain of human erythroid beta spectrin (repeats 13-15) in complex with the spectrin binding domain of human erythroid ankyrin (ZU5-ANK), EMTS derivative
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-07 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 90.130, 98.540, 137.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.820 - 2.750 |
R-factor | 0.22797 |
Rwork | 0.225 |
R-free | 0.27724 |
Structure solution method | SAD |
Starting model (for MR) | PDB entries 3F57 3f59 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.644 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.870 |
High resolution limit [Å] | 2.750 | 2.750 |
Number of reflections | 32570 | |
<I/σ(I)> | 16 | 4.4 |
Completeness [%] | 99.8 | 100 |
Redundancy | 4.9 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 283 | Protein complex at 7.5 mg/mL mixed 1:1 with 0.05 M MES pH 6.5, 0.2 M ammonium acetate, 0.01 M calcium chloride, 10% PEG-4000, VAPOR DIFFUSION, HANGING DROP, temperature 283K |