3ISW
Crystal structure of filamin-A immunoglobulin-like repeat 21 bound to an N-terminal peptide of CFTR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-01 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 74.152, 74.152, 289.063 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.730 - 2.800 |
| R-factor | 0.264 |
| Rwork | 0.263 |
| R-free | 0.29800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2brq |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.644 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK (9.7LDz) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.970 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.071 | 0.472 |
| Number of reflections | 12452 | |
| <I/σ(I)> | 13.8 | 3.3 |
| Completeness [%] | 96.6 | 98.5 |
| Redundancy | 8.53 | 10.41 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M Bis-Tris Propane pH 7.0, 60% Tacsimate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
