3II7
Crystal structure of the kelch domain of human KLHL7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-06-25 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54180 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.435, 50.905, 87.500 |
| Unit cell angles | 90.00, 113.19, 90.00 |
Refinement procedure
| Resolution | 28.340 - 1.630 |
| R-factor | 0.1614 |
| Rwork | 0.161 |
| R-free | 0.18996 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vpj |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.336 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.340 | 1.720 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.083 | 0.636 |
| Number of reflections | 38407 | |
| <I/σ(I)> | 13.8 | 2.6 |
| Completeness [%] | 99.2 | 98.8 |
| Redundancy | 6.5 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.15 | 12% PEG 20000, 0.1M MES pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |
