3HQU
PHD2:Fe:UN9:partial HIF1-alpha substrate complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.29 |
| Spacegroup name | P 63 |
| Unit cell lengths | 110.819, 110.819, 39.615 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.550 - 2.300 |
| Rwork | 0.188 |
| R-free | 0.22200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2g19 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.068 | 0.312 |
| Number of reflections | 12581 | |
| <I/σ(I)> | 16.658 | 3.3 |
| Completeness [%] | 99.1 | 96.2 |
| Redundancy | 3.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 6.5 | 298 | 1.6M AMMONIUM SULFATE, 4% DIOXANE, 0.1M MES PH6.5, ARGON ATMOSPHERE, 20MG/ML PROTEIN WITH 1MM FE(II)SO4, 10MM HIF1A-CODD-OH PEPTIDE, hanging drop, temperature 298K |
