3H06
Crystal structure of the binding domain of the AMPA subunit GluR2 bound to the willardiine antagonist, UBP282
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-01-01 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.977 |
Spacegroup name | P 1 |
Unit cell lengths | 91.013, 90.916, 92.510 |
Unit cell angles | 85.61, 85.52, 72.40 |
Refinement procedure
Resolution | 39.710 - 2.800 |
Rwork | 0.230 |
R-free | 0.29480 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.102 | 0.372 |
Number of reflections | 64109 | |
<I/σ(I)> | 1.84 | 1.84 |
Completeness [%] | 96.7 | 79 |
Redundancy | 2.4 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 15-17% PEG8K, 0.2 M Na acetate, 0.2 M Na cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |