3FVK
Crystal structure of the human glutamate receptor, GluR5, ligand-binding core in complex with 8-deoxy-neodysiherbaine A in space group P1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-11-25 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.508, 50.787, 62.644 |
| Unit cell angles | 80.43, 84.41, 62.10 |
Refinement procedure
| Resolution | 30.000 - 1.500 |
| R-factor | 0.16727 |
| Rwork | 0.166 |
| R-free | 0.19148 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fvn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.242 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 78332 | |
| <I/σ(I)> | 35.9 | 3.2 |
| Completeness [%] | 96.0 | 87.4 |
| Redundancy | 3.8 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 303 | 35% PEG3350, 0.3M LiSO4, 5mM 8-deoxy-neodysiherbaine, pH5.5, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
