3F9K
Two domain fragment of HIV-2 integrase in complex with LEDGF IBD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9802 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 201.360, 202.500, 280.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.990 - 3.200 |
| R-factor | 0.22538 |
| Rwork | 0.225 |
| R-free | 0.23421 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2B4J (chains A and C) 1K6Y (chain A residue 1-43) |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.948 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.161 | 0.562 |
| Number of reflections | 188411 | |
| <I/σ(I)> | 13 | 3.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.9 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 2.6M sodium acetate, 10mM MgCl2, 0.1M Bis-Tris Propane-HCl, pH7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
