3EVO
Crystal structure of the Mimivirus NDK +Kpn mutant complexed with dTDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2007-06-26 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.931 |
Spacegroup name | P 63 |
Unit cell lengths | 69.759, 69.759, 103.262 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.907 - 1.500 |
R-factor | 0.161 |
Rwork | 0.160 |
R-free | 0.18500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b8p |
RMSD bond length | 0.007 |
RMSD bond angle | 1.336 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.580 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 43329 | |
<I/σ(I)> | 11.9 | 3 |
Completeness [%] | 95.2 | 74.6 |
Redundancy | 6 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 1.1M sodium citrate, 0.1M Tris, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |