3EQY
Crystal structure of human MDMX in complex with a 12-mer peptide inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-05-27 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 31 |
Unit cell lengths | 75.627, 75.627, 35.181 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 1.630 |
R-factor | 0.15551 |
Rwork | 0.155 |
R-free | 0.16874 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2z5t |
RMSD bond length | 0.019 |
RMSD bond angle | 1.746 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.510 | 1.690 |
High resolution limit [Å] | 1.630 | 1.630 |
Rmerge | 0.045 | 0.101 |
Number of reflections | 27972 | |
<I/σ(I)> | 64.3 | 20.2 |
Completeness [%] | 99.9 | 94.8 |
Redundancy | 5.2 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M HEPES, 0.8 M sodium phosphate, 0.8 M potassium phosphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |