3EGI
Methyltransferase domain of human trimethylguanosine synthase TGS1 bound to m7GpppA (inactive form)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, Hamburg |
Beamline | BW7A |
Temperature [K] | 130 |
Detector technology | CCD |
Collection date | 2006-06-05 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9799, 0.9801 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 213.893, 213.893, 62.415 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.900 - 2.210 |
Rwork | 0.210 |
R-free | 0.25200 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.291 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.048 | |
Number of reflections | 71001 | |
Completeness [%] | 97.0 | |
Redundancy | 8.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293 | Na-Formate, pH 6.10, VAPOR DIFFUSION, HANGING DROP, temperature 293K |