3DO4
Crystal structure of transthyretin variant T60A at acidic pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9729 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.586, 75.907, 107.267 |
| Unit cell angles | 90.00, 93.10, 90.00 |
Refinement procedure
| Resolution | 107.200 - 2.400 |
| R-factor | 0.20517 |
| Rwork | 0.202 |
| R-free | 0.26743 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f41 |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.215 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 107.200 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.069 | 0.486 |
| Number of reflections | 38055 | |
| <I/σ(I)> | 14.3 | 2.9 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 298 | 15% PEG 4000, 0.05M NaAcetate, 0.1M AmmoniumAcetate , pH 4.6, VAPOR DIFFUSION, temperature 298K |
