3D4B
Crystal structure of Sir2Tm in complex with Acetyl p53 peptide and DADMe-NAD+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 77.4 |
| Detector technology | CCD |
| Collection date | 2005-07-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.790, 59.735, 106.114 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.1972 |
| Rwork | 0.195 |
| R-free | 0.22948 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 3.431 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.100 | 0.370 |
| Number of reflections | 23509 | |
| Completeness [%] | 99.4 | 96.54 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 292 | PEG 3350, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
