3CX8

Crystal Structure of PDZRhoGEF rgRGS Domain in a Complex with Galpha-13 Bound to GTP-gamma-S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-26
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	58.109, 66.117, 152.521
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.000
R-factor	0.243
Rwork	0.223
R-free	0.25300
Starting model (for MR)	1zcb 1htj
RMSD bond length	0.007
RMSD bond angle	1.306
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	40370
<I/σ(I)>	22.9	2.3
Completeness [%]	98.1	90.7
Redundancy	5.2	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	17-24 % polyethylene glycol 8000 and 100 mM Na+ HEPES, pH 7.3-7.8, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K