3C6M
Crystal structure of human spermine synthase in complex with spermine and 5-methylthioadenosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ DW |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-07-12 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 32 |
| Unit cell lengths | 88.262, 88.262, 197.668 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.650 - 2.450 |
| R-factor | 0.2117 |
| Rwork | 0.209 |
| R-free | 0.27092 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c6k |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.478 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.450 |
| Number of reflections | 61292 |
| Completeness [%] | 97.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 300 | 18% PEG 3350, 0.1 M NaCl, 0.1 M Bis-Tris pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
