3BJI

Structural Basis of Promiscuous Guanine Nucleotide Exchange by the T-Cell Essential Vav1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-01
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.95
Spacegroup name	P 1 21 1
Unit cell lengths	78.948, 75.079, 114.857
Unit cell angles	90.00, 103.87, 90.00

Refinement procedure

Resolution	30.000 - 2.600
R-factor	0.22473
Rwork	0.221
R-free	0.29253
Structure solution method	SAD
Starting model (for MR)	1foe
RMSD bond length	0.013
RMSD bond angle	1.825
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.667
High resolution limit [Å]	2.600	2.600
Number of reflections	36156
Completeness [%]	100.0	100
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	17% PEG-3350, 100 mM HEPES, pH 7.5, 200 mM ammonium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 298K