3BJI
Structural Basis of Promiscuous Guanine Nucleotide Exchange by the T-Cell Essential Vav1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.95 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.948, 75.079, 114.857 |
| Unit cell angles | 90.00, 103.87, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.22473 |
| Rwork | 0.221 |
| R-free | 0.29253 |
| Structure solution method | SAD |
| Starting model (for MR) | 1foe |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.825 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.667 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 36156 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 17% PEG-3350, 100 mM HEPES, pH 7.5, 200 mM ammonium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
