3BJI
Structural Basis of Promiscuous Guanine Nucleotide Exchange by the T-Cell Essential Vav1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-01 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.95 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 78.948, 75.079, 114.857 |
Unit cell angles | 90.00, 103.87, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.600 |
R-factor | 0.22473 |
Rwork | 0.221 |
R-free | 0.29253 |
Structure solution method | SAD |
Starting model (for MR) | 1foe |
RMSD bond length | 0.013 |
RMSD bond angle | 1.825 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.667 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 36156 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 17% PEG-3350, 100 mM HEPES, pH 7.5, 200 mM ammonium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 298K |