3BDX
Crystal structure of the unstable and highly fibrillogenic Pro7Ser mutant of the Recombinant variable domain 6AJL2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-09-07 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 56.110, 106.120, 133.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.920 - 2.300 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b5g |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.920 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.084 | 0.227 |
| Number of reflections | 16902 | |
| <I/σ(I)> | 8.7 | 3.9 |
| Completeness [%] | 93.9 | |
| Redundancy | 2.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | Drops consisted of 5 microliters of protein solution (7 mg/mL) plus 5 microliters of 0.1 M MES pH 6.5, 2.0 M Sodium acetate trihydrate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
