3ULU
Structure of quaternary complex of human TLR3ecd with three Fabs (Form1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-03 |
| Detector | RIGAKU SATURN 944 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 214.900, 142.080, 125.040 |
| Unit cell angles | 90.00, 103.17, 90.00 |
Refinement procedure
| Resolution | 45.075 - 3.520 |
| R-factor | 0.2701 |
| Rwork | 0.268 |
| R-free | 0.30910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2A0Z 1ziw 3uls 3na9 3qpq |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.611 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_896)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.700 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.202 | 0.682 |
| Number of reflections | 34547 | |
| <I/σ(I)> | 8.4 | 2.2 |
| Completeness [%] | 74.1 | 12.6 |
| Redundancy | 5.3 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 293 | 0.1 M sodium acetate, pH 4.5, 28% PEG3350, 1 M lithium chloride, 30 mM Glycyl-glycyl-glycine, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
