3SDJ
Structure of RNase-inactive point mutant of oligomeric kinase/RNase Ire1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2009-11-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.115879 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 158.790, 163.450, 298.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.652 - 3.650 |
| R-factor | 0.2505 |
| Rwork | 0.248 |
| R-free | 0.28730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.027 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.652 |
| High resolution limit [Å] | 3.650 |
| Number of reflections | 86573 |
| Completeness [%] | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 300 | SODIUM CITRATE, pH 5.6, VAPOR DIFFUSION, temperature 300K |
