3S9D
binary complex between IFNa2 and IFNAR2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-05 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.650, 62.010, 88.080 |
| Unit cell angles | 90.00, 92.68, 90.00 |
Refinement procedure
| Resolution | 19.897 - 2.000 |
| Rwork | 0.204 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3S8W 2hym and 1RH2 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.897 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 61371 | |
| <I/σ(I)> | 17.6 | 2.6 |
| Completeness [%] | 99.1 | 99.1 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 293 | 20% (w/v) PEG 3350, 200 mM NaSCN, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
