3RGF
Crystal Structure of human CDK8/CycC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-05-10 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.690, 70.610, 79.140 |
| Unit cell angles | 90.00, 108.31, 90.00 |
Refinement procedure
| Resolution | 37.567 - 2.200 |
| R-factor | 0.1826 |
| Rwork | 0.180 |
| R-free | 0.22090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1ZP2 2i53 2pk2 2WH2 2pk2 2rk3 2uze 2r3q 2r3n 2vth |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.004 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.567 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.089 | 0.542 |
| Number of reflections | 37725 | |
| <I/σ(I)> | 10.7 | 2.6 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 4.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG3350, 0.2M lithium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
