3QS9
Crystal structure of a human Flt3 ligand-receptor ternary complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-01 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 124.750, 153.550, 133.870 |
Unit cell angles | 90.00, 94.57, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 8.000 |
High resolution limit [Å] | 7.800 | 7.800 |
Rmerge | 0.128 | 0.807 |
Number of reflections | 5656 | |
<I/σ(I)> | 9.08 | 1.9 |
Completeness [%] | 96.6 | 92.9 |
Redundancy | 3.38 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 12-14% w/v PEG3350, 0.2 M lithium citrate, 0.1 M TRIS-HCl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |