3QE9
Crystal structure of human exonuclease 1 Exo1 (D173A) in complex with DNA (complex I)
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 92.504, 95.467, 99.777 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.510 |
| Rwork | 0.215 |
| R-free | 0.23769 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.843 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.780 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 27346 | |
| Completeness [%] | 93.3 | 62.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | PEG6K, HEPES, CaCl2, KCl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG6K | ||
| 2 | 1 | 1 | HEPES | ||
| 3 | 1 | 1 | CaCl2 | ||
| 4 | 1 | 1 | KCl | ||
| 5 | 1 | 2 | PEG6K | ||
| 6 | 1 | 2 | HEPES | ||
| 7 | 1 | 2 | CaCl2 | ||
| 8 | 1 | 2 | KCl |
