3PSE
Structure of a viral OTU domain protease bound to interferon-stimulated gene 15 (ISG15)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-06 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 34.740, 69.810, 69.670 |
Unit cell angles | 90.00, 93.62, 90.00 |
Refinement procedure
Resolution | 34.671 - 2.300 |
R-factor | 0.1666 |
Rwork | 0.164 |
R-free | 0.22400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1Z2M AND 3PT2 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.944 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.900 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.107 | 0.566 |
Number of reflections | 14495 | |
<I/σ(I)> | 7.4 | 2.3 |
Completeness [%] | 97.8 | 98 |
Redundancy | 3.1 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | 100 mM MES, 23% PEG6000, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |