3PFV
Crystal structure of Cbl-b TKB domain in complex with EGFR pY1069 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9763 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.063, 98.934, 61.978 |
Unit cell angles | 90.00, 110.63, 90.00 |
Refinement procedure
Resolution | 49.470 - 2.270 |
R-factor | 0.21861 |
Rwork | 0.216 |
R-free | 0.26201 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 3BUO |
RMSD bond length | 0.015 |
RMSD bond angle | 1.567 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.470 | 2.390 |
High resolution limit [Å] | 2.270 | 2.270 |
Rmerge | 0.090 | 0.604 |
Number of reflections | 30186 | |
<I/σ(I)> | 8 | 2.1 |
Completeness [%] | 96.6 | 96.6 |
Redundancy | 3.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.15 | 30% PEG 3350, 0.3M Ammonium sulfate, 0.1M Bis-Tris, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |